EMPIAR-13982 - Cryo-EM dataset of human UAP56–LENG8–PCID2–SEM1 complex in the presence of 15U RNA and ATP [multiple data sets in MRC format]

EMPIAR-13982

Cryo-EM dataset of human UAP56–LENG8–PCID2–SEM1 complex in the presence of 15U RNA and ATP [multiple data sets in MRC format]

Publication:

Molecular basis of polyadenylated RNA fate determination in the nucleus

Bugai A, Hohmann U, Lorenzo A, Graf M, Fin L, Rouvière JO, Tirian L, Dou Y, Le Rest M, Polák P, Johnsen D, Jakobsen L, Andersen JS, Brennecke J, Plaschka C, Jensen TH

Nature 655 (2026) 1070-1078

PMID: 42310446

DOI: 10.1038/s41586-026-10650-0

Related EMDB entry:

EMD-56931 EMD-56932

Deposited:

2026-09-15

Released:

2026-10-07

Last modified:

2026-10-07

Imageset size:

822.28 GB

Imageset DOI:

10.6019/EMPIAR-13982

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Dose-weighted motion-corrected micrographs of the engineered human LENG8(491–800)–PCID2–UAP56–N-UBM–SEM1 complex with 15U RNA and ATP

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6578

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.749, 0.749)

Details:

Dose-weighted 4096 x 4096 micrographs of 10xHis-MBP-LENG8(491-800), 3xV5-PCID2-full-length-UAP56-N-UBM fusion, and SEM1 sample incubated with 15U RNA and ATP. Micrographs were generated by CryoSPARC patch motion correction from EER movies decoded into 40 fractions. Acquisition: Titan Krios, Falcon 4i, 300 kV, spherical aberration 2.7 mm, total dose 50 e-/A^2.

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2. Non-dose-weighted motion-corrected micrographs of the engineered human LENG8(491–800)–PCID2–UAP56–N-UBM–SEM1 complex with 15U RNA and ATP

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6578

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.749, 0.749)

Details:

Non-dose-weighted 4096 x 4096 micrographs of 10xHis-MBP-LENG8(491-800), 3xV5-PCID2-full-length-UAP56-N-UBM fusion, and SEM1 sample incubated with 15U RNA and ATP. Micrographs were generated by CryoSPARC patch motion correction from EER movies decoded into 40 fractions. Acquisition: Titan Krios, Falcon 4i, 300 kV, spherical aberration 2.7 mm, total dose 50 e-/A^2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Burton Smith RN, Murata K. (2026)

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Watanabe R, Lee EB. (2026)

Ligand-induced activation of RyR1 in native membranes.

Mikirtumov V, Golusik S, Huo R, Sprink T, Balyschew N, Yang W, Diebolder C, Yuan S, Kotecha A, Kudryashev M. (2026)

QwenCryoMarker: a universal post-processing framework for contamination-aware particle cleaning.

Sun Y, Zhao J, Xu N, Wang L, Ding W, Li M. (2026)

CryoMV: Structure-Prior-Guided Modeling and Real-Particle Validation of Continuous Conformational Transitions in Cryo-EM

Li F, Dong H, Tang S, Wang X, Zhang C, Ji C, Han R, Zhang F, Wan X. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

See all citations

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