EMPIAR-14023 - Cryo-EM structure of DDM1-HELLS chimera bound to the nucleosome [7808 multi-frame micrographs composed of 40 frames each in EER format]

EMPIAR-14023

Cryo-EM structure of DDM1-HELLS chimera bound to the nucleosome [7808 multi-frame micrographs composed of 40 frames each in EER format]

Publication:

Cryo-EM complex of DDM1-HELLS chimera bound to the nucleosome

Nartey W, Williams GJ

(---)

Related EMDB entry:

EMD-40569 EMD-40563 EMD-40562

Deposited:

2026-07-16

Released:

2026-10-16

Last modified:

2026-10-16

Imageset size:

3.45 TB

Imageset DOI:

10.6019/EMPIAR-14023

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of DDM1-HELLS chimera bound to the nucleosome

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7808

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.77, 0.77)

Details:

7808 raw EER movies (Thermo Fisher Falcon 4, EPU, 4096 x 4096) used to obtain EMD-40569 and focused maps EMD-40562 and EMD-40563. Collected at 300 kV, Cs 2.7 mm, pixel size 0.77 A/px, total exposure 50 e-/A^2. EER data were fractionated into 40 frames during processing in cryoSPARC 3.3.2. Gain reference: data/movies/gain/20220217_121244_EER_GainReference.gain (EPU-generated Falcon 4 EER gain reference, used as supplied by EPU). Movies are split into subdirectories data/movies/batch_01 to batch_04 (max 2000 files each).

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Citations

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