EMPIAR-13613 - Single particle cryo-EM dataset of Leishmania tarentolae step II trans-spliceosome [multiple data sets in TIFF format]

EMPIAR-13613

Single particle cryo-EM dataset of Leishmania tarentolae step II trans-spliceosome [multiple data sets in TIFF format]

Publication:

Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites

Nadenoen T, Biglione FA, Vandevenne M, Vanden Broeck A

Nature communications 17 (2026)

PMID: 42778629

DOI: 10.1038/s41467-026-77480-6

Related EMDB entry:

EMD-52843 EMD-52844 EMD-52795 EMD-52806 EMD-52794 EMD-52796 EMD-52798 EMD-52803 EMD-52801 EMD-52805

Deposited:

2026-02-07

Released:

2026-11-04

Last modified:

2026-11-04

Imageset size:

22.46 TB

Imageset DOI:

10.6019/EMPIAR-13613

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Leishmania tarentolae step II trans-spliceosome - Dataset 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

21415

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 50.0 e-/Å2 (40 frames). The gain reference was applied on-the-fly during data collection.

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2. Unaligned multi-frame micrographs of Leishmania tarentolae step II trans-spliceosome - Dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10577

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.54, 0.54)

Details:

The dataset was collected on a Titan Krios electron microscope operating at 300 kV equipped with an energy filter (slit width 20 eV), a Cs corrector and a K3 Summit detector set in super-resolution mode. Unaligned multi-frame micrographs were recorded at a nominal magnification of 64,000× with a pixel size of 0.54 Å/pixel, a defocus range of -0.5 to -2.5 µm, and a total dose of 59.0 e-/Å2 (50 frames). The gain reference was applied on-the-fly during data collection.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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