EMPIAR-13172 - Cryogenic transmission electron microscopy images of extracellular vesicles isolated from conditioned media of pancreatic cancer and normal pancreatic epithelial cell lines [3 micrographs in TIFF format]

EMPIAR-13172

Cryogenic transmission electron microscopy images of extracellular vesicles isolated from conditioned media of pancreatic cancer and normal pancreatic epithelial cell lines [3 micrographs in TIFF format]

Publication:

Extracellular vesicle derived miRNA-182-5p educates macrophages towards an immunosuppressive phenotype in pancreatic cancer

Singh BS, Gaur PG, Bose PB, Zhang YJ, Li YL, Zhang ZZ, Kandhavelu JK, Klotzbier WK, Jayatilake MJ, Bansal SB, Farhan MF, Deol SD, Banerjee PPB, Unger KU, Gupta SG, Verma VV, Cheema AKC

Signal Transduction and Targeted Therapy (---)

Related EMDB entry:

Deposited:

2025-12-13

Released:

2025-12-24

Last modified:

2025-12-24

Imageset size:

96.07 MB

Imageset DOI:

10.6019/EMPIAR-13172

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Extracellular vesicles (EVs) isolated from PANC-1, PPCL-68, and hTERT-HPNE cell lines conditioned media

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(3.82, 3.82)

Details:

EV samples (∼3.5 μL) were loaded onto glow-discharged, perforated carbon-coated grids (2/1-3C C-Flat; Protochips, Raleigh, NC), blotted with filter paper, and rapidly plunged into liquid ethane. These grids were stored in liquid nitrogen. Before imaging, the grids were placed on cryo-specimen holder (626 cryo-specimen holder, Gatan, Warrendale, PA) and maintained at -180°C. The digital micrographs were captured using a Tecnai F20 Twin transmission electron microscope (ThermoFisher Scientific, Hillsboro, OR) equipped with a Gatan US4000 CCD or a Teitz XF416 camera.

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Citations

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

The vault associates with membranes in situ.

Geißler K, Kreysing JP, Wang Y, Glushkova D, Obarska-Kosinska A, Hoffmann PC, Böhm S, Schmidt A, Meier-Credo J, Langer JD, Hummer G, Beck M. (2026)