EMPIAR-12962 - Cryo-EM micrographs of PbAbiF dimer bound to ncRNA [4272 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12962

Cryo-EM micrographs of PbAbiF dimer bound to ncRNA [4272 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins

Zilberzwige-Tal S, Altae-Tran H, Kannan S, Wilkinson ME, Vo SC, Strebinger D, Edmonds KK, Yao CJ, Mears KS, Shmakov SA, Makarova KS, Macrae RK, Koonin EV, Zhang F

Cell 188 (2025) 1925-1940.e20

PMID: 39970912

DOI: 10.1016/j.cell.2025.01.034

Related EMDB entry:

EMD-43682

Deposited:

2025-09-09

Released:

2025-12-07

Last modified:

2025-12-07

Imageset size:

587.76 GB

Imageset DOI:

10.6019/EMPIAR-12962

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of PbAbiF RNP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4272

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.663, 0.663)

Details:

Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM

Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.663 A/pix
Dose 42.2 e/A2
Exposure rate 18.5 e/pix/s
Exposure time 1.00 s
Fractions 40

Total mics 4272

GainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC.

particles.star contains metadata for the particle selected for the final reconstruction.

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Citations

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)