EMPIAR-12699 - Cryo-EM structure of spMETTL16 in complex with U6 snRNA_delta17 [6009 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12699

Cryo-EM structure of spMETTL16 in complex with U6 snRNA_delta17 [6009 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structures and mechanisms of U6 snRNA m<sup>6</sup>A modification by METTL16

Ju J, Tomita K

Nature communications 16 (2025)

PMID: 40841561

DOI: 10.1038/s41467-025-63021-0

Related EMDB entry:

EMD-63833

Deposited:

2025-04-11

Released:

2026-03-06

Last modified:

2026-03-06

Imageset size:

1.34 TB

Imageset DOI:

10.6019/EMPIAR-12699

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM structure of spMETTL16 in complex with U6 snRNA_delta17

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6009

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Spherical aberration (Cs): 2.7
- Nominal Defocus (nm): -800 to -1800
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Please use gain.mrc as a gain reference.
- In CryoSPARC, select "flip gain ref in Y".

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