EMPIAR-12616 - Cryo-EM of SARS-CoV-2 pre-incorporation replication-transcription complex with UTP [30850 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12616

Cryo-EM of SARS-CoV-2 pre-incorporation replication-transcription complex with UTP [30850 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural basis for substrate selection by the SARS-CoV-2 replicase

Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Götte M, Chait BT, Campbell EA, Darst SA

Nature 614 (2023) 781-787

PMID: 36725929

DOI: 10.1038/s41586-022-05664-3

Related EMDB entry:

EMD-26642

Deposited:

2025-03-07

Released:

2025-04-24

Last modified:

2025-04-24

Imageset size:

8.92 TB

Imageset DOI:

10.6019/EMPIAR-12616

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

30850

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.08, 1.08)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 21.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 52 e-/A^2; pixel size 1.08 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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