EMPIAR-12328 - RNAP-TopoI complex on short-overhang scaffold [multiple data sets in TIFF and MRCS formats]

EMPIAR-12328

RNAP-TopoI complex on short-overhang scaffold [multiple data sets in TIFF and MRCS formats]

Publication:

DNA topoisomerase I acts as supercoiling sensor for transcription elongation in E. coli

Vidmar VV, Borde CB, Bruno LB, Takacs MT, Batisse CB, Saint-Andre CSA, Zhu CZ, Espeli OE, Lamour VL, Weixlbaumer AW

(---)

Related EMDB entry:

EMD-51271 EMD-51270

Deposited:

2024-10-03

Released:

2025-11-18

Last modified:

2025-11-18

Imageset size:

1.49 TB

Imageset DOI:

10.6019/EMPIAR-12328

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - unprocessed

Image Sets

1. unaligned multi-frame movies of RNAP-TopoI on short-overhang scaffold

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7254

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.862, 0.862)

Details:

300 kV, Cs 0.01 mm, defocus range -1.0 – -2.0 um, total dose 55.55 e/A2
Gain reference file is provided in data/movies/gain.mrc

Files:

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2. picked particles of RNAP-TopoI on short-overhang scaffold

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

7250

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.862, 0.862)

Details:

Particles were picked and extracted in WARP (Tegunov & Cramer, Nat. Methods 2019) as a part of on-the-fly processing pipeline at the IGBMC, Strasbourg, France.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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