EMPIAR-12324
CryoEM of E. coli RNA polymerase on lambda PR promoter DNA [3511 multi-frame micrographs composed of 30 frames each in TIFF format]
Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

Related EMDB entry:
Deposited:
2024-10-04
Released:
2024-12-05
Last modified:
2024-12-05
Imageset size:
1.04 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3511
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.70 e-/ A^2/s; frame time 0.2 s (30 frames) with total exposure of 6 s; total dose 46.2 e-/A^2; pixel size 1.06 A/px(detector); pixel size 0.53 A/px(image); defocus range -0.9 to -2.4 um; gain reference 18jul13c_17105458_05_7676x7420_norm_0_mod.mrc in references folder.
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