EMPIAR-12261 - Cryo electron tomography of Enterovirus 71-infected cells [49 tilt series in MRC format]

EMPIAR-12261

Cryo electron tomography of Enterovirus 71-infected cells [49 tilt series in MRC format]

Publication:

Endosome rupture enables enteroviruses to infect cells

Ishemgulova A, Mukhamedova L, Trebichalská Z, Rájecká V, Payne P, Šmerdová L, Moravcová J, Hrebík D, Buchta D, Škubník K, Füzik T, Vaňáčová Š, Nováček J, Plevka P

Communications Biology (---)

Related EMDB entry:

EMD-15711 EMD-15710

Deposited:

2024-09-04

Released:

2024-11-21

Last modified:

2024-11-21

Imageset size:

22.65 GB

Imageset DOI:

10.6019/EMPIAR-12261

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo electron tomography of Enterovirus 71-infected cos-7 cells

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Tilt series:
- pixel width and height: 5.532 Å
- Number of tilts: 49 (Enterovirus71_30mpi_tomo7, Enterovirus71_60mpi_tomo12, Enterovirus71_60mpi_tomo16, Enterovirus71_60mpi_tomo18), 41 (Enterovirus71_60mpi_tomo24, Enterovirus71_60mpi_tomo26, Enterovirus71_60mpi_tomo8)
- number of columns, rows: 3838, 3710
- dose-symmetric tilt scheme;
± 48°, 2° tilt increment (Enterovirus71_30mpi_tomo7, Enterovirus71_60mpi_tomo12, Enterovirus71_60mpi_tomo16, Enterovirus71_60mpi_tomo18), ± 50°, 2.5° tilt increment (Enterovirus71_60mpi_tomo24, Enterovirus71_60mpi_tomo26, Enterovirus71_60mpi_tomo8)
- rawtlt, angles file available
Tomograms:
- pixel width and height: 11.06 Å (Enterovirus71_30mpi_tomo7, Enterovirus71_60mpi_tomo12, Enterovirus71_60mpi_tomo16, Enterovirus71_60mpi_tomo18), 16.60 Å (Enterovirus71_60mpi_tomo24, Enterovirus71_60mpi_tomo26, Enterovirus71_60mpi_tomo8)

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

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Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

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Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM.

Kung JE, Johnson MC, Tegunov D, Jao CC, Wu P, Oh A, Lin M, Daria JM, Koth CM, Arthur CP, Rohou A, Sudhamsu J. (2025)

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