EMPIAR-12190 - FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate [5595 micrographs in TIFF format]

EMPIAR-12190

FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate [5595 micrographs in TIFF format]

Publication:

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen en Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, Hoogenboom JP

Methods in Microscopy (---)

Related EMDB entry:

Deposited:

2024-06-20

Released:

2024-09-23

Last modified:

2024-09-23

Imageset size:

602.65 GB

Imageset DOI:

10.6019/EMPIAR-12190

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Raw FAST-EM array tomography electron micrographs from wild-type MCF-7 cells stained with rOTO protocol and uranyl acetate

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

5595

Image size:

(6400, 6400)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(40.0, 40.0)

Details:

Raw serial-section (array tomography) data of MCF-7 cells acquired with FAST-EM, ordered per section. Each section directory (SXX) contains 10x10 images covering an area of approximately 240 x 240 microns, with 6.25% overlap between images. Images were acquired with 20 us dwell time.

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Citations

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Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

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Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM.

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