EMPIAR-12175 - Cryo-ET dataset of dormant microsporidian spores from Encephalitozoon hellem [multiple data sets in MRCS and MRC formats]

EMPIAR-12175

Cryo-ET dataset of dormant microsporidian spores from Encephalitozoon hellem [multiple data sets in MRCS and MRC formats]

Publication:

Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites

Kelley K, Raczkowski AM, Klykov O, Jaroenlak P, Bobe D, Kopylov M, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ, Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC

Proceedings of the National Academy of Sciences of the United States of America 122 (2025)

PMID: 40067903

DOI: 10.1073/pnas.2415233122

Related EMDB entry:

Deposited:

2024-06-10

Released:

2025-05-22

Last modified:

2025-05-22

Imageset size:

270.71 GB

Imageset DOI:

10.6019/EMPIAR-12175

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Aligned tilt series from microsporidian Encephalitozoon hellem

Category:

tilt series

Image format:

MRCS

No. of images or tilt series:

Image size:

(4200, 4200)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(3.298, 3.298)

Details:

Each tilt series in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tilt_series where the corresponding aligned tilt series and tilt file can be found.

Files:

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2. Reconstructed tomograms from microsporidian Encephalitozoon hellem

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.298, 3.298)

Details:

Each reconstructed tomogram in this dataset exists in a unique directory with an identifiable ID. Within each directory is a folder called tomo_rec where the 2X binned reconstruction produced using protomo can be found.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)

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