EMPIAR-12146
Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system

Feng Z, Yashiro Y, Tomita K

Nucleic acids research (2024)

PMID: 39228374

Related EMDB entry:
Deposited:
2024-06-20
Released:
2024-09-26
Last modified:
2024-09-26
Imageset size:
1.69 TB
Imageset DOI:
Experimental metadata:
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Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Imageset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7044
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (Å/pix): 0.83
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (nm): -800 to -1800
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 50
- Please use gain.mrc as a gain reference.
- In CryoSPARC, select "flip gain ref in Y".
Files:
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