EMPIAR-12130 - In situ cryo-electron tomograms #1-#2 of human brain organoid from SOLIST procedure. [multiple data sets in MRC format]

EMPIAR-12130

In situ cryo-electron tomograms #1-#2 of human brain organoid from SOLIST procedure. [multiple data sets in MRC format]

Publication:

Serialized on-grid lift-in sectioning for tomography (SOLIST) enables a biopsy at the nanoscale

Nguyen HTD, Perone G, Klena N, Vazzana R, Kaluthantrige Don F, Silva M, Sorrentino S, Swuec P, Leroux F, Kalebic N, Coscia F, Erdmann PS

Nature methods 21 (2024) 1693-1701

PMID: 39271806

DOI: 10.1038/s41592-024-02384-6

Related EMDB entry:

EMD-50607 EMD-50608

Deposited:

2024-06-11

Released:

2025-01-23

Last modified:

2025-01-23

Imageset size:

5.50 GB

Imageset DOI:

10.6019/EMPIAR-12130

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Motion-corrected tilt series of human brain organoid using SOLIST procedure.

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.953, 1.953)

Details:

Tilt series images are mirrored (opposite handedness) with respect to final average.

Files:

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2. Motion-corrected tilt series of human brain organoid using SOLIST procedure.

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.953, 1.953)

Details:

Tilt series images are mirrored (opposite handedness) with respect to final average.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)

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