EMPIAR-12108
Cryogenic electron microscopy of blot-plunged acetyl-CoA decarbonylase/synthase [14078 multi-frame micrographs composed of 21 frames each in TIFF format]
Publication:

Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy

Biester A, Grahame DA, Drennan CL

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 39361653

Related EMDB entry:
Deposited:
2024-05-28
Released:
2025-01-23
Last modified:
2025-01-23
Imageset size:
1.97 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
14078
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.832, 0.832)
Details:
Gain reference:
data/GainsBackup2021-12-10/K3-18030019 Gain Ref.x1.m1.bin2.mrc
Defocus range: -0.8 to -2.1 µM
Total exposure on specimen: 54.53 el/A2
Exposure time: 1.69 s
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3
Files:
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