EMPIAR-12078 - Single particle dataset of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs plus Fluorinated Octyl Maltoside (FOM) [4561 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12078

Single particle dataset of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs plus Fluorinated Octyl Maltoside (FOM) [4561 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structure of the turnover-ready state of an ancestral respiratory complex I

Ivanov BS, Bridges HR, Jarman OD, Hirst J

Nature communications 15 (2024)

PMID: 39472559

DOI: 10.1038/s41467-024-53679-3

Related EMDB entry:

EMD-18324 EMD-18325

Deposited:

2024-04-11

Released:

2025-01-20

Last modified:

2025-01-20

Imageset size:

2.06 TB

Imageset DOI:

10.6019/EMPIAR-12078

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of respiratory complex I from Paracoccus dentrificans in MSP2N2 nanodiscs plus Fluorinated Octyl Maltoside (FOM)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4561

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

Non-gain corrected micrographs were imaged using a Gatan K3 in super-resolution mode and with a GIF BioQuantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 4.0 s exposures captured in 40 frames and a total dose of 45 electrons Å-2. Dataset also contains polished particle stacks and gain reference files.

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Citations

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