EMPIAR-11844
Cryo-EM of Tetrahymena ribozyme [multiple data sets in TIFF and MRC formats]
Publication:

Complex Water Networks Visualized through 2.2-2.3 Angstrom Cryogenic Electron Microscopy of RNA

Kretsch RC, Li S, Pintilie G, Palo MZ, Case DA, Das R, Zhang K, Chiu W

bioRxiv (2025)

Related EMDB entry:
Deposited:
2023-11-28
Released:
2025-02-04
Last modified:
2025-02-04
Imageset size:
4.01 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs of tetrahymena ribozyme.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
18366
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.82, 0.82)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a K3 detector. Over the collection, the sample was exposed evenly to 57.25 electrons per angstrom squared with a total exposure time of 2.5 seconds. Desired defocuses ranged from -0.5 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/ThR/exposures/K3-18210041 Gain Ref. x1.m1.mrc.
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2. Extracted particles and orientations for final reconstruction of tetrahymena ribozyme.
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
1181000
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.82, 0.82)
Details:
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/ThR/particles/2.2A_cryosparc_P5_J1222_004_particles.cs and data/ThR/particles/2.3A_cryosparc_P5_J1224_004_particles.cs
Files:
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