EMPIAR-11830 - In situ cryo-ET dataset of Chlamydomonas reinhardtii prepared using cryo-plasmaFIB milling [multiple data sets in EER and MRC formats]

EMPIAR-11830

In situ cryo-ET dataset of Chlamydomonas reinhardtii prepared using cryo-plasmaFIB milling [multiple data sets in EER and MRC formats]

Publication:

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii

Kelley R, Khavnekar S, Righetto RD, Heebner J, Obr M, Zhang X, Chakraborty S, Tagiltsev G, Michael AK, van Dorst S, Waltz F, McCafferty CL, Lamm L, Zufferey S, Van der Stappen P, van den Hoek H, Wietrzynski W, Harar P, Wan W, Briggs JA, Plitzko JM, Engel BD, Kotecha A

bioRxiv (2024)

DOI: 10.1101/2024.12.28.630444

Related EMDB entry:

EMD-18306

Deposited:

2024-04-09

Released:

2024-04-09

Last modified:

2024-04-10

Imageset size:

29.45 TB

Imageset DOI:

10.6019/EMPIAR-11830

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Chlamy Visual proteomics project containing 1829 tilt series and preprocessed metadata for C. reinhardtii prepared using cryo-plasmaFIB milling

Category:

tilt series

Image format:

EER

No. of images or tilt series:

1829

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.96, 1.96)

Details:

A Minimal TOMOMAN (https://doi.org/10.1093/micmic/ozad067.516) project.

Files:

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2. Chlamy Visual proteomics denoised tomograms

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

1829

Image size:

(1024, 1024)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(7.84, 7.84)

Details:

Denoised using CryoCARE by using workflow implemented in TOMOMAN

Files:

This entry cannot be downloaded from PDBj.

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3. Chlamy Visual proteomics 3D CTF-corrected tomograms

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

1829

Image size:

(1024, 1024)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(7.84, 7.84)

Details:

3D CTF-corrected tomograms calculated with IMOD.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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