EMPIAR-10953 - Apoferritin on EG-grid [7500 multi-frame micrographs composed of 64 frames each in TIFF format]

EMPIAR-10953

Apoferritin on EG-grid [7500 multi-frame micrographs composed of 64 frames each in TIFF format]

Publication:

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis

Fujita J, Makino F, Asahara H, Moriguchi M, Kumano S, Anzai I, Kishikawa J, Matsuura Y, Kato T, Namba K, Inoue T

Scientific reports 13 (2023)

PMID: 36755111

DOI: 10.1038/s41598-023-29396-0

Related EMDB entry:

EMD-31314

Deposited:

2021-11-12

Released:

2023-02-28

Last modified:

2023-02-28

Imageset size:

903.09 GB

Imageset DOI:

10.6019/EMPIAR-10953

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Apoferritin on EG-grid

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7500

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.49, 0.49)

Details:

Unaligned and not gain corrected 64 frame movies with an accumulated exposure of 40 electrons per squared Angstrom. The gain reference was generated from a part of the dataset with "relion_estimate_gain" program in RELION 3.1.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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