EMPIAR-10489
Native Pyruvate Dehydrogenase Complex from Neurospora crassa [4887 micrographs in MRC format]
Publication:

Arrangement and symmetry of the fungal E3BP-containing core of the pyruvate dehydrogenase complex

Forsberg BO, Aibara S, Howard RJ, Mortezaei N, Lindahl E

Nature communications 11 (2020)

PMID: 32938938

Related EMDB entry:
Deposited:
2020-07-04
Released:
2020-09-02
Last modified:
2021-05-07
Imageset size:
328.03 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Aligned and dose-weighted micrographs of native N. crassa Pyruvate dehydrogenase
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
4887
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.25, 1.25)
Details:
All motion-corrected, dose-weighted and gain-corrected micrographs are contained in the directory "MG".

No extracted particles are included. Instead, particles can be re-extracted using the .star files. Estimated CTF values per-micrograph is found in the file micrographs_ctf.star, which should be used to re-extract particles defined in any particles.star-file. The latter files contain refined positions and angle assignments.

all_particles.star contain all particles considered to be non-junk, PDC-particles. Angle assignments are based on classification of these particles into class X, class Y, and class N, as denoted in the paper "Arrangement and symmetry of the fungal E3BP-containing core of the Pyruvate Dehydrogenase Complex". Two separate files contain the re-refined angles of class X and Yi respectively, based on gold-standard refinement using relion.
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