EMPIAR-13398
C. thermocellum UvrA in complex with DNA with a fluorescein modification and AMPPNP [multiple data sets in TIFF and MRCS formats]
Publication:

Structural snapshots of the mechanism of ATP-dependent DNA damage recognition by UvrA

Nirwal S, Czarnocki-Cieciura M, Zajko W, Skowronek K, Szczepanowski RH, Nowotny M

Nature communications 17 (2025)

PMID: 41381534

Related EMDB entry:
Deposited:
2026-03-15
Released:
2026-03-27
Last modified:
2026-03-27
Imageset size:
2.24 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame movies of C. thermocellum UvrA in complex with DNA with a fluorescein modification and AMPPNP
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10348
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.84, 0.84)
Details:
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU) with 0 + 30 deg stage tilt. Voltage: 300 kV; spherical aberration: 2.7; dose per frame: ~1 e/A^2 (total dose: 40.54 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.1, -1.8, -1.5, -1.2, -0.9, -0.6 / -2.5, -2.0, -1.5 µm. Objective aperture retracted, C2 aperture: 50 µm. See microscope_settings.txt for details
Files:
Loading...
2. Aligned particles of C. thermocellum UvrA in complex with DNA with a fluorescein modification and AMPPNP (ATP-bound conformation 1)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
126432
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.84, 0.84)
Details:
Aligned particles with corresponding STAR file. Micrographs were initially binned 2x during Motion correction.
Files:
Loading...
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
He L, Bartesaghi A. (2026)
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Jones HN, Deshmukh A, Pande K. (2026)
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)
Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)
Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)
Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)