EMPIAR-13288
cryo electron tomography of mouse hippocampal CA1 stratum radiatum - dataset 4 [multiple data sets in EER and MRC formats]
Publication:

A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue

Glynn C, Smith JLR, Case M, Csöndör R, Katsini A, Sanita ME, Glen TS, Pennington A, Grange M

Cell reports methods 5 (2025)

PMID: 40527314

Related EMDB entry:
Deposited:
2026-02-10
Released:
2026-03-12
Last modified:
2026-03-12
Imageset size:
342.30 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
  • reconstructed volumes
1. raw eer of tilt series
Category:
tilt series
Image format:
EER
No. of images or tilt series:
66
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.98, 1.98)
Details:
66 tilt series comprised of 41 tilts with 3 degree spacing between tilts in eer format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel.
Files:
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2. raw .mrc of tilt series
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
66
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.98, 1.98)
Details:
66 tilt series comprised of 41 tilts with 3 degree spacing between tilts in mrc format. Total dose 130e-/A^2 / tilt series for 3.17e-/A^2 / tilt. Data acquired at 64000x with 1.98A / pixel.
Files:
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3. unfiltered bin 8 reconstructions
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
66
Image size:
(512, 512)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(15.84, 15.84)
Details:
Unfiltered nondenoised reconstructed tomograms from 66 tilt series carried out in aretomo2.
Files:
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4. isonet deconvolved bin8 tomograms
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
64
Image size:
(512, 512)
Pixel type:
BIT
Pixel spacing:
(15.84, 15.84)
Details:
64 unfiltered reconstructed tomograms deconvolved (but not missing wedge corrected) in isonet, bin8. --snrfalloff 0.7 --deconvstrength 1.1.
Files:
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