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EMPIAR-12953
CryoEM movies of the human mortalin(R126W)-GrpEL1(WT) complex [multiple data sets in EER format]
Publication:

Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70

Morizono MA, McGuire KL, Birouty NI, Herzik MA

Nature communications 15 (2024) 10815-10815

PMID: 39737924

Related EMDB entry:
EMD-44675
Deposited:
2025-08-28
Released:
2025-09-17
Last modified:
2025-09-17
Imageset size:
5.11 TB
Imageset DOI:
10.6019/EMPIAR-12953
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 1
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
952
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.935, 0.935)
Details:
Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

Gain file required for motion correction of unaligned micrographs for dataset 1 is included.
Files:
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2. Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 2
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
1757
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.935, 0.935)
Details:
Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

Gain file required for motion correction of unaligned micrographs for dataset 2 is included.
Files:
Loading...
3. Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex - dataset 3
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
1861
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.935, 0.935)
Details:
Unaligned multi-frame micrographs of the human mortalin(R126W)-GrpEL1(WT) complex collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 130,000x in EF-TEM mode (with a calibrated pixel size of 0.935 Å) on a Falcon-4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.

Gain file required for motion correction of unaligned micrographs for dataset 3 is included.
Files:
Loading...
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