Single-particle cryo-EM of Staphylococcus aureus ribosomes inhibited by fusidic acid and fusidic acid cyclopentane [multiple data sets in EER, TIFF and MRC formats]
Publication:
Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane
González-López A, Larsson DSD, Koripella RK, Cain BN, Chavez MG, Hergenrother PJ, Sanyal S, Selmer M
Data (raw movies) were collected at SciLifeLab in Umeå, Sweden in a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at 165,000 x nominal magnification with a calibrated pixel size of 0.728 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 520 nm beam size. The EER formatted movies were collected in 684 raw frames with a total dose of 27.77 e-/Å2 (12.45 e-/pixel/s) over 2.23 s with a set defocus between -0.7 to -1.3 µm. The movies are not gain-corrected, use the file provided within the main folder.
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Data (raw movies) were collected at SciLifeLab in Solna, Sweden on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at 105,000 x nominal magnification with a calibrated pixel size of 0.824 Å. A 50 µm C2 aperture was inserted and the TEM was operated in nanoprobe mode at spot size 6 and 670 nm beam size. The movies were collected in 30 frames over 1.3 s with a total dose of 30.83 e-/Å2 (16.1 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm. The movies are gain-corrected.
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Raw movies from the FA-CP inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 31 frames over 28.74 s with a total dose of 29.8 e-/Å2 (0.94 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm. The movies are gain corrected.
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Raw movies from the FA inhibited ribosomes screening in a Glacios microscope operated at 200 kV with a magnification of 150,000x with a pixel size of 0.952 Å/pix. The movies were collected in 30 frames over 27.79 s with a total dose of 30 e-/Å2 (1.00 e-/pixel/s) with a set defocus between -0.5 to -1.5 µm. The movies are gain corrected.
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