EMPIAR-11946 - Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells [multiple data sets in MRC format]

EMPIAR-11946

Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells [multiple data sets in MRC format]

Publication:

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Khusainov I, Romanov N, Goemans C, Turoňová B, Zimmerli CE, Welsch S, Langer JD, Typas A, Beck M

Nature communications 15 (2024)

PMID: 38839776

DOI: 10.1038/s41467-024-49084-5

Related EMDB entry:

EMD-18036 EMD-18038

Deposited:

2024-03-07

Released:

2024-06-19

Last modified:

2024-06-19

Imageset size:

38.47 GB

Imageset DOI:

10.6019/EMPIAR-11946

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 1

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.697, 1.697)

Details:

The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -64 to +48 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.

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2. Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 2

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.697, 1.697)

Details:

The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -63 to +45 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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