EMPIAR-11814
Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5c extended [multiple data sets in TIFF and MRC formats]
Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

Related EMDB entry:
Deposited:
2023-11-28
Released:
2024-05-13
Last modified:
2024-05-13
Imageset size:
1.11 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 with SL5c extended RNA.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5880
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.68, 0.68)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV, using a BioQuantum energy filter with slit size 20eV, and a K3 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 1.19 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/SARS-CoV-2_SL5-6_5cext/exposures/K3-18210041 Gain Ref. x1.m1.mrc.
Files:
Loading...
2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 with SL5c extended RNA.
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
65937
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.04, 2.04)
Details:
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6_5cext/particles/P3_J1245_particles_exported.cs.
Files:
Loading...