EMPIAR-11247 - Cryo-EM structure of the RhlR-PqsE complex from Pseudomonas aeruginosa [multiple data sets in TIFF and MRCS formats]

EMPIAR-11247

Cryo-EM structure of the RhlR-PqsE complex from Pseudomonas aeruginosa [multiple data sets in TIFF and MRCS formats]

Publication:

Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation

Feathers JR, Richael EK, Simanek KA, Fromme JC, Paczkowski JE

Structure (London, England : 1993) 30 (2022) 1626-1636.e4

PMID: 36379213

DOI: 10.1016/j.str.2022.10.008

Related EMDB entry:

EMD-27646 EMD-27645

Deposited:

2022-08-03

Released:

2023-01-19

Last modified:

2023-01-19

Imageset size:

2.18 TB

Imageset DOI:

10.6019/EMPIAR-11247

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of RhlR-PqsE complex from Pseudomonas aeruginosa

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2357

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.645, 0.645)

Details:

Raw super-resolution movies of 60 frames saved in tif format.

Files:

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2. Polished particles of RhlR-PqsE complex from Pseudomonas aeruginosa

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

2229

Image size:

(168, 168)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.29, 1.29)

Details:

Polished particle image stacks used to generate EMD27645 maps

Files:

Archives and downloads the selected files into an uncompressed zip file.

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3. Polished particles of RhlR-PqsE complex from Pseudomonas aeruginosa bound to DNA

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1909

Image size:

(168, 168)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.29, 1.29)

Details:

Polished particle image stacks used to generate EMD27646 maps

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Kim K, Li H, Clarke OB. (2025)

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM.

Kung JE, Johnson MC, Tegunov D, Jao CC, Wu P, Oh A, Lin M, Daria JM, Koth CM, Arthur CP, Rohou A, Sudhamsu J. (2025)

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