EMPIAR-10860 - In situ cryo-electron tomography of E. coli APEC 2248 infected by Goslar [1073 tilt series in TIFF format]

EMPIAR-10860

In situ cryo-electron tomography of E. coli APEC 2248 infected by Goslar [1073 tilt series in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25229 EMD-25360 EMD-25358 EMD-25359 EMD-25262

Deposited:

2021-11-07

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

56.04 GB

Imageset DOI:

10.6019/EMPIAR-10860

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unaligned tilt-movie frames as unormalized LZW-TIFF

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

1073

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.265, 4.265)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 CW rotation prior to application to TIFs. Fluence per tilt-movie is 1.8 electrons per square Angstrom. SerialEM metadata for the 19 tilt-series is in data/mdocs, Warp/M metadata in data/xmls_and_tomostars, and positions of ribosomes in data/ribosome_positions as M formatted .star files.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)

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