EMPIAR-10772
Cryo-electron tomography of rod outer segments in wild type mice acquired with Volta Phase Plate in focus [2 reconstructed volumes in MRC format]
Publication:

Determinants shaping the nanoscale architecture of the mouse rod outer segment

Pöge M, Mahamid J, Imanishi SS, Plitzko JM, Palczewski K, Baumeister W

eLife 10 (2021)

PMID: 34931611

Related EMDB entry:
Deposited:
2021-07-21
Released:
2022-01-07
Last modified:
2022-01-07
Imageset size:
3.20 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
1. Tomograms of rod outer segments in wild type mice acquired with Volta phase plate
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
2
Image size:
(928, 928)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(10.48, 10.48)
Details:
4x binned and dose filtered tomograms with dimensions 928x928x464. Tilt series were acquired conventionally with defocus. Projection images were recorded between -50° and 50° with an increment of 2°. The 314 frames are distributed over all projections. The total exposure per tilt-series was ~ 100e-/A^2.
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