EMPIAR-10025 - T20S Proteasome at 2.8 Å Resolution [multiple data sets in MRC format]

EMPIAR-10025

T20S Proteasome at 2.8 Å Resolution [multiple data sets in MRC format]

Publication:

2.8 Angstrom resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy.

Campbell MG, Veesler D, Cheng A, Potter CS, Carragher B

Elife 4 (2015) e06380-e06380

PMID: 25760083

DOI: doi:10.7554/eLife.06380

Related EMDB entry:

EMD-6287

Deposited:

2015-02-26

Released:

2015-03-06

Last modified:

2015-03-06

Imageset size:

1.97 TB

Imageset DOI:

10.6019/EMPIAR-10025

Experimental metadata:

Download xml json

Version history:

2015-07-01

REL

Added Relion star file with coordinates: data/14sep05c_raw_196/run1_shiny_mp007_data_dotstar.txt

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Raw movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

196

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.66, 0.66)

Details:

Super-resolution data-acquisition. dark-amibox05-0.mrc and norm-amibox05-0.mrc are used for gain correction.
run1_shiny_mp007_data_dotstar.txt is a Relion star file with particle coordinates etc.

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Frame-averaged micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

196

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.66, 0.66)

Details:

Super-resolution data-acquisition.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Aligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

196

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.66, 0.66)

Details:

Super-resolution data-acquisition.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)