EMPIAR-12443
Single particle reconstruction of human apo TRPM8 channel in a closed state [multiple data sets in TIFF and MRCS formats]
Publication:

Structure of human TRPM8 channel

Palchevskyi S, Czarnocki-Cieciura M, Vistoli G, Gervasoni S, Nowak E, Beccari AR, Nowotny M, Talarico C

Communications biology 6 (2023)

PMID: 37857704

Related EMDB entry:
Deposited:
2024-10-29
Released:
2025-02-03
Last modified:
2025-02-03
Imageset size:
3.69 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame movies of human apo TRPM8 channel in a closed state
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7918
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.82, 0.82)
Details:
Gain-corrected, unaligned multi-frame movies collected with Krios G3i microscope + K3 detector (superresolution mode, binned 2x with EPU). Voltage: 300 kV; spherical aberration: 2.7; dose per frame: 1 e/A^2 (total dose: 41.42 e/A^2 fractionated over 40 frames). Defocus range (nominal): -2.7, -2.4, -2.1, -1.8, -1.5, -1.2, -0.9 µm. Objective aperture retracted, C2 aperture: 50 µm.
Files:
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2. Aligned particles of human apo TRPM8 channel in a closed state (consensus map)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
110176
Image size:
(420, 420)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.82, 0.82)
Details:
Aligned particles with corresponding Relion-3 STAR file for the consensus TRPM8 reconstruction. Coordinates correspond to micrographs binned 2x during Motion correction.
Files:
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3. Aligned particles of human apo TRPM8 channel in a closed state (focused pre-MHR + MHR1/2 map)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
212851
Image size:
(192, 192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.82, 0.82)
Details:
Aligned particles with corresponding Relion-3 STAR file for the focused TRPM8 reconstruction (pre-MHR + MHR1/2 map). Particles were signal-subtracted; coordinates correspond to micrographs binned 2x during Motion correction.
Files:
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