SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface [stack of 230000 particles in MRCS format]
Publication:
SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface
Esfahani BG, Randolph PS, Peng R, Grant T, Stroupe ME, Stagg SM
The particle stack is the picked lipid nanotube particles. The 3D volume is the tube azimuthal average to be subtracted from the picked particles for further processing based on the described protocol in the SPOT-RASTR paper.
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