EMPIAR-11681
Cryo-EM structure of yeast SWR1C subunit Swc5 bound to the nucleosome [multiple data sets in TIFF and MRC formats]
Publication:

Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C

Baier AS, Gioacchini N, Eek P, Leith EM, Song T, Peterson CL

eLife (---)

Related EMDB entry:
Deposited:
2023-09-05
Released:
2024-06-06
Last modified:
2024-06-06
Imageset size:
6.53 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned multi-frame micrographs of Swc5/nucleosome complex
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13855
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.394, 0.394)
Details:
Voltage 300 keV, Cs 2.7 mm, total dose 50 e-/Å^2, super-resolution pixel size 0.394 Å/px. The TIFF movies are not gain-corrected, the gain-reference image for the dataset is SuperRef_stack_00000_X+0Y-1-1.dm4 and gain.mrc.
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2. High-resolution particles obtained after filtering with random-phase 3D classification
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
974026
Image size:
(216, 216)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.182, 1.182)
Details:
Selected particles obtained after multiple rounds of heterogenous refinement against a low-pass filtered reconstruction. Particles were extracted from motion-corrected micrographs that were f-cropped by factor of 1/2. Extraction box size 324 px Fourier cropped to 216 px. These were used for final 3D classification and refinement. The 3D classes are listed as star-files in data/classes.
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