Single particle cryo-EM dataset, collected with a 20 degree tilt, of Bos taurus mitochondrial complex I (originally purified in LMNG) proteoliposomes and deactivated [4550 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:
Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I
Unaligned multi-frame micrographs of wild-type bovine mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to liposomes. Not gain corrected (gain file included). The proteoliposomes were heat treated (37 ºC, 20 min) to deactivate the sample, before being frozen on graphene oxide gold grids. Images were collected with a 20º tilt. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Gatan K3 detector in super-resolution mode but bin2 at the hardware stage, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 3.8 s exposures were captured in 40 frames with a total dose of ~40 Å-2. A pixel size of 1.072 Å/pixel was used.
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