EMPIAR-11349 - Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease [9524 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11349

Cryo-EM structure of a delivery complex containing the SspB adaptor, an ssrA-tagged substrate, and the AAA+ ClpXP protease [9524 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

The SspB adaptor drives structural changes in the AAA⁺ClpXP protease during ssrA-tagged substrate delivery

Ghanbarpour A, Fei X, Baker TA, Davis JH, Sauer RT

bioRxiv (2022)

DOI: 10.1101/2022.11.06.515074

Related EMDB entry:

EMD-28585

Deposited:

2022-11-30

Released:

2023-01-31

Last modified:

2023-01-31

Imageset size:

4.42 TB

Imageset DOI:

10.6019/EMPIAR-11349

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned Movies of a Delivery Complex Containing the SspB Adaptor, an ssrA-Tagged Substrate, and the AAA+ ClpXP Protease

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9524

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.435, 0.435)

Details:

Nominal Defocus range (min, max, step): -1.0, -2.5, -0.25
Total exposure on specimen: 75.98 el/A2
Exposure time: 2.02 s
Nominal pixel size: 0.87 A (0.435 A super res)
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3

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