EMPIAR logo

Electron Microscopy Public Image Archive

EMPIAR-12319
CryoEM of E. coli RNA polymerase on T7A1 promoter DNA [6790 multi-frame micrographs composed of 30 frames each in TIFF format]
Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

Related EMDB entry:
EMD-23897
Deposited:
2024-10-03
Released:
2024-12-21
Last modified:
2024-12-21
Imageset size:
1.72 TB
Imageset DOI:
10.6019/EMPIAR-12319
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6790
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.24 e-/ A^2/s; pixel size 1.06 A/px(detector); pixel size 0.53 A/px(image); exposure 6 s; frame time 0.2 s; total dose 43.45 e-/A^2; total frames 30; defocus range -1.1 to -2.4 um; gain reference file 18nov08d_08110340_01_7676x7420_norm_0_mod.mrc is in references folder
Files:
Loading...
Browse All Files
Quick Links
Browse EMPIAR
Sample Preparation Widget
Volume Browser
Tomo Viewer Prototype
Claim entries to your ORCiD
Talks and Tutorials
EMPIAR Quick tour
Publications
Re-use study
Statistics
EMPIAR in the news
Contact us
COVID-19 Data Portal
EMDB
BioImage Archive
PDBe
EMNavigator@PDBj
PDBj
Citations
High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles
Kim K, Li H, Clarke OB. (2025)
Self-supervised learning for generalizable particle picking in cryo-EM micrographs.
Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)
In situ structure determination of conformationally flexible targets using nextPYP.
Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)
CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.
Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)
Few-shot learning for non-vitrified ice segmentation.
Vivas-Lago A, Castaño-Díez D. (2025)
Automated removal of corrupted tilts in cryo-electron tomography.
Majtner T, Turoňová B. (2025)
Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.
Felix J, De Munck S, Bazan JF, Savvides SN. (2025)
Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.
Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)
Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)
Molecular exaptation by the integrin αI domain.
Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)
See all citations
Protein Data Bank Japan News
[2025-09-17]
231件のPDBエントリーが新たに公開されました。（2025-09-17付）
[2025-08-24]
第63回日本生物物理学会年会にてランチョンセミナーを開催します
[2025-08-10]
IIBMP2025にてランチョンセミナーを開催します
[2025-07-17]
[wwPDB] PDBx/mmCIFフォーマットと拡張PDB IDへの移行について
[2025-06-25]
[wwPDB] EMDBアクセションコードを拡張する予定です
[2025-06-05]
第25回日本蛋白質科学会年会にてランチョンセミナーを開催します
[2025-04-22]
[wwPDB] PDBアーカイブのスナップショットは、HTTPS、SYNC、RSYNC、FTP経由でダウンロード頂けます
[2025-03-25]
PDBj Newsletter Vol.25公開のお知らせ
[2025-03-11]
[wwPDB] 生物物理学会年会にてポスター賞を授与しました
[2025-03-10]
[wwPDB] 新しいバージョンのPDB-IHM 検証レポートに、クロスリンキング質量分析に基づく構造モデルの評価が追加されました。
See all news