EMPIAR-11855
β2-adrenergic receptor in complex with nucleotide-free G protein Gs [multiple data sets in TIFF format]
Publication:

Time-resolved cryo-EM of G-protein activation by a GPCR

Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi M, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G

Nature (2024)

PMID: 38480881

Deposited:
2024-01-08
Released:
2024-05-14
Last modified:
2024-05-14
Imageset size:
1.50 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of nucleotide-free β2-adrenergic receptor in complex with G protein Gs (Set 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6446
Image size:
(3710, 3838)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
Raw unaligned movie files (tif format, 40 frames, not gain corrected). Collected on a Titan Krios equipped with a K2 Summit direct electron detector and post-column energy filter. The microscope was operated at 300 kV accelerating voltage. Magnified pixel size of 1.06Å. Μovies were obtained at an exposure of 1.3 electrons/Å^2/frame.
Files:
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2. Unaligned multi-frame micrographs of nucleotide-free β2-adrenergic receptor in complex with G protein Gs (Set 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1169
Image size:
(3710, 3838)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
Raw unaligned movie files (tif format, 40 frames, not gain corrected). Collected on a Titan Krios equipped with a K2 Summit direct electron detector and post-column energy filter. The microscope was operated at 300 kV accelerating voltage. Magnified pixel size of 1.06Å. Μovies were obtained at an exposure of 1.3 electrons/Å^2/frame.
Files:
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