Voltage 300 keV, Cs 2.7 mm, total dose 50 e-/Å^2, super-resolution pixel size 0.394 Å/px. The TIFF movies are not gain-corrected, the gain-reference image for the dataset is SuperRef_stack_00000_X+0Y-1-1.dm4 and gain.mrc.
Selected particles obtained after multiple rounds of heterogenous refinement against a low-pass filtered reconstruction. Particles were extracted from motion-corrected micrographs that were f-cropped by factor of 1/2. Extraction box size 324 px Fourier cropped to 216 px. These were used for final 3D classification and refinement. The 3D classes are listed as star-files in data/classes.