micrographs - multiframe

MRC

2038

(5760, 4092)

UNSIGNED BYTE

(0.899, 0.899)

Data collected on 9/17/2020-9/18-2020
Krios, K3, counted mode, EFTEM, Cs corrector
User/Group:Brian Caldwell/Chuck Bell

Image information:
Total # of images = 2038
Total data size = 1.70 TB
Rate of collection = 71/hour
Mode of collection = Accurate hole centering
Gain normalized = YES
File format = mrc
Super-resolution = NO
Spherical aberration = 0.01 mm
Images per hole = 1
Exposure time = 2.7 s
Fractions = 36 [usually 1-2 electrons per fractions]

Microscope parameters:
Pixel size = 0.899 A
Magnification = 81,000
Stage tilt = 0 deg
Dose rate = 19.644 e/px/s
Dose rate = 24.28 e/A^2/s
Total dose = 65 e/A^2 [desired dose rate]
Defocus range = -3.5, -3, -2.5, -2, -1.5, -1
C2 aperture = 50 um
Objective aperture= 100 um
Illuminated area = 1.5 um
Energy Filter = YES
Slit width = 20 eV

Grid type = Quantifoil (gold coated) R1.2/1.3 300 Mesh hole size, mesh, etc.
Position 1 of the large dewar Bell Conical: Position 1 of box J2 (Gray)
Prepared on 8/20/2020 by Brian Caldwell

Additional notes:
Copied gain reference files over into the data folder.
Saved EPU settings file.

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