EMPIAR-11306
Cryo-electron tomography on plasma FIB lamellae of HeLa cells [6481 tilt series in EER format]
Publication:

Plasma FIB milling for the determination of structures in situ

Berger C, Dumoux M, Glen T, Yee NB, Mitchels JM, Patáková Z, Darrow MC, Naismith JH, Grange M

Nature communications 14 (2023)

PMID: 36746945

Related EMDB entry:
Deposited:
2022-11-10
Released:
2023-02-03
Last modified:
2023-02-03
Imageset size:
668.07 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • tilt series
1. Tilt-series recorded on argon PFIB lamellae of HeLa cells
Category:
tilt series
Image format:
EER
No. of images or tilt series:
6481
Image size:
(4096, 4096)
Pixel type:
4 BIT INTEGER
Pixel spacing:
(1.9, 1.9)
Details:
Tilt-series recorded on argon PFIB lamellae of HeLa cells using a dose-symmetric tilt-scheme corrected for the pre-tilt of the lamellae. Electron dose: 5 e/A2 per tilt. Data recorded at a pixel size of 1.85 A/px, which was refined to 1.9A/px based on the Subtomogram average structure.
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