EMPIAR-11199
Thermostabilized human prestin in complex with chloride, sulfate or salicylate [multiple data sets in TIFF format]
Publication:

Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility

Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O

Nature communications 13 (2022)

PMID: 36266333

Related EMDB entry:
Deposited:
2022-06-28
Released:
2023-04-14
Last modified:
2023-04-14
Imageset size:
3.86 TB
Imageset DOI:
Experimental metadata:
Download xml json
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Cl bound
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
9361
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata1-3_3-A-001_0000_Feb22_11.46.30.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.
Files:
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2. Sulfate bound
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
8155
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata4-1_36-A-001_0000_Mar05_13.25.01.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.
Files:
Loading...
3. Salicylate bound
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6751
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata3-2_69-6_0000_Mar03_17.09.30.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.
Files:
Loading...
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