EMPIAR-10874
Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets) [multiple data sets in MRCS, MRC and EER formats]
Publication:

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7

Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL

Life science alliance 5 (2022)

PMID: 35794005

Related EMDB entry:
Deposited:
2021-11-08
Released:
2022-09-05
Last modified:
2022-09-05
Imageset size:
13.97 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
  • picked particles - multiframe - processed
1. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
1957
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.7512, 0.7512)
Details:
Movies collected using Tundra. 24 frames were used, pixel size is 0.7512 Å/pixel, total dose of 40 e-/Å2. Compressed using pbzip2.
Files:
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2. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1957
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.7512, 0.7512)
Details:
Micrographs collected using Tundra. Aligned and dose weighted using UCSF MotionCor2.
Files:
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3. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.461, 1.461)
Details:
Final particle stack after particle polishing using Relion.
Files:
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4. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
5142
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 36 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
Files:
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5. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5142
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
Files:
Loading...
6. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(360, 360)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Final particle stack after particle polishing using Relion.
Files:
Loading...
7. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
2013
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 37 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
Files:
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8. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2013
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
Files:
Loading...
9. Polished particles of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Final particle stack after particle polishing using Relion.
Files:
Loading...
10. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
5498
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 44 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
Files:
Loading...
11. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5498
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
Files:
Loading...
12. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Final particle stack after particle polishing using Relion.
Files:
Loading...
13. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
2468
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 41 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.
Files:
Loading...
14. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2468
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
Files:
Loading...
15. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(360, 360)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3225, 1.3225)
Details:
Final particle stack after particle polishing using Relion.
Files:
Loading...
16. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
3029
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.895, 0.895)
Details:
Movies collected using Glacios with Selectris X and Falcon 4. EER format was used, pixel size is 0.895 Å/pixel, total dose of 40 e-/Å2.
Files:
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17. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3029
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.895, 0.895)
Details:
Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.
Files:
Loading...
18. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.56625, 1.56625)
Details:
Final particle stack after particle polishing using Relion.
Files:
Loading...