EMPIAR logo

Electron Microscopy Public Image Archive

EMPIAR-10818
Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]
Publication:

Structural basis of substrate recognition by a polypeptide processing and secretion transporter

Kieuvongngam V, Olinares PDB, Palillo A, Oldham ML, Chait BT, Chen J

eLife 9 (2020)

PMID: 31934861

Related EMDB entry:
EMD-21132
Deposited:
2021-09-10
Released:
2021-11-26
Last modified:
2021-11-26
Imageset size:
2.00 TB
Imageset DOI:
10.6019/EMPIAR-10818
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned and uncorrected multiframe movies of PCAT1 bound to its CtA peptide substrate
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3879
Image size:
(7420, 7676)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.545, 0.545)
Details:
The micrographs are the raw 50-frame tif movies collected with a super-resolution pixel size 0.545 Angstrom (1.09 Angstrom after binning). The dataset is separated into two subdirectories. The mrc movies has been compressed to bz2 format. The gain reference files are in a separate directory as the movie datasets
Files:
Loading...
Browse All Files
Quick Links
Browse EMPIAR
Sample Preparation Widget
Volume Browser
Tomo Viewer Prototype
Claim entries to your ORCiD
Talks and Tutorials
EMPIAR Quick tour
Publications
Re-use study
Statistics
EMPIAR in the news
Contact us
COVID-19 Data Portal
EMDB
BioImage Archive
PDBe
EMNavigator@PDBj
PDBj
Citations
High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles
Kim K, Li H, Clarke OB. (2025)
Self-supervised learning for generalizable particle picking in cryo-EM micrographs.
Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)
In situ structure determination of conformationally flexible targets using nextPYP.
Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)
CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.
Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)
Few-shot learning for non-vitrified ice segmentation.
Vivas-Lago A, Castaño-Díez D. (2025)
Automated removal of corrupted tilts in cryo-electron tomography.
Majtner T, Turoňová B. (2025)
Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.
Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)
Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.
Felix J, De Munck S, Bazan JF, Savvides SN. (2025)
Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)
Molecular exaptation by the integrin αI domain.
Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)
See all citations
Protein Data Bank Japan News
[2025-09-17]
231件のPDBエントリーが新たに公開されました。（2025-09-17付）
[2025-08-24]
第63回日本生物物理学会年会にてランチョンセミナーを開催します
[2025-08-10]
IIBMP2025にてランチョンセミナーを開催します
[2025-07-17]
[wwPDB] PDBx/mmCIFフォーマットと拡張PDB IDへの移行について
[2025-06-25]
[wwPDB] EMDBアクセションコードを拡張する予定です
[2025-06-05]
第25回日本蛋白質科学会年会にてランチョンセミナーを開催します
[2025-04-22]
[wwPDB] PDBアーカイブのスナップショットは、HTTPS、SYNC、RSYNC、FTP経由でダウンロード頂けます
[2025-03-25]
PDBj Newsletter Vol.25公開のお知らせ
[2025-03-11]
[wwPDB] 生物物理学会年会にてポスター賞を授与しました
[2025-03-10]
[wwPDB] 新しいバージョンのPDB-IHM 検証レポートに、クロスリンキング質量分析に基づく構造モデルの評価が追加されました。
See all news