EMPIAR-12598 - Cryo electron microscopy of backtracked SARS-CoV-2 nsp13-replication-transcription complex [10685 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12598

Cryo electron microscopy of backtracked SARS-CoV-2 nsp13-replication-transcription complex [10685 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex

Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33883267

DOI: 10.1073/pnas.2102516118

Related EMDB entry:

EMD-23008 EMD-23007 EMD-23009

Deposited:

2025-02-27

Released:

2025-04-24

Last modified:

2025-04-24

Imageset size:

4.36 TB

Imageset DOI:

10.6019/EMPIAR-12598

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10685

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.065, 1.065)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 30 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 26.4 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 66 e-/A^2; pixel size 1.065 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.

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Citations

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Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

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