Dataset 1. Super-resolution movies (binned 2x during motion correction). Total dose = 45.6 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_02222023/gainref.
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Dataset 2. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_03092023/gainref.
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Dataset 3. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04112023/gainref.
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Dataset 4. Super-resolution movies (binned 2x during motion correction). Total dose = 43.7 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04122023/gainref.
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Dataset 5. Super-resolution movies (binned 2x during motion correction). Total dose = 44 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_07022023/gainref.
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PLEASE NOTE: This dataset was not used to generate the associated EMDs and PDBs. This dataset was collected under different sample preparations conditions (Mg-F-actin instead of Ca-G-actin) to demonstrate F-actin severing by gelsolin. The grids used for this dataset were also different (2nm ultra-thin carbon), resulting in a minor orientation bias. Please see methods and Extended Data Fig. 4 in the publication (doi.org/10.1038/s41594-024-01412-5) for more details.
Super-resolution movies (binned 2x during motion correction). Total dose = 45.3 electrons/Å^2. The gain reference is also provided as a .mrc file in data/Beckman_04052024/gainref.
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