EMPIAR-12174 - FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate [688 micrographs in TIFF format]

EMPIAR-12174

FAST-EM array tomography data of rat pancreas prepared with rOTO protocol and stained with neodymium acetate [688 micrographs in TIFF format]

Publication:

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen En Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, Hoogenboom JP

Methods in microscopy 1 (2024) 49-64

PMID: 39119255

DOI: 10.1515/mim-2024-0005

Related EMDB entry:

Deposited:

2024-06-20

Released:

2024-08-29

Last modified:

2024-08-29

Imageset size:

74.86 GB

Imageset DOI:

10.6019/EMPIAR-12174

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Raw FAST-EM array tomography electron micrographs from rat pancreas tissue with rOTO staining and neodymium acetate

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

688

Image size:

(6400, 6400)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(40.0, 40.0)

Details:

Raw FAST-EM images ordered per section. Each section (SXX) is represented by a mosaic of 4 by 4 micrographs, with filenames corresponding to the index in the mosaic (XXX-YYY-0.tif)

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Citations

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Kim K, Li H, Clarke OB. (2025)

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In situ structure determination of conformationally flexible targets using nextPYP.

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Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

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Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

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