EMPIAR-11441 - FMC63 scFv in complex with soluble CD19 [multiple data sets in TIFF format]

EMPIAR-11441

FMC63 scFv in complex with soluble CD19 [multiple data sets in TIFF format]

Publication:

CD19 CAR antigen engagement mechanisms and affinity tuning

He C, Mansilla-Soto J, Khanra N, Hamieh M, Bustos V, Paquette AJ, Garcia Angus A, Shore DM, Rice WJ, Khelashvili G, Sadelain M, Meyerson JR

Science immunology 8 (2023)

PMID: 36867678

DOI: 10.1126/sciimmunol.adf1426

Related EMDB entry:

EMD-26719

Deposited:

2023-01-16

Released:

2023-03-17

Last modified:

2023-03-17

Imageset size:

1.59 TB

Imageset DOI:

10.6019/EMPIAR-11441

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. FMC63-CD19 dataset1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

489

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.548, 0.548)

Details:

Arctica operated at 200 kV with a GatanK3 imaging system collected at 36,000X nominal magnification. The calibrated pixel size of 0.5480 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 21.17 e-/Å2/s with a total exposure of 2.40 seconds, for an accumulated dose of 50.82 e-/Å2. Intermediate frames were recorded every 0.05 seconds for a total of 48 frames per micrograph.

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2. FMC63-CD19 dataset2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1910

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.548, 0.548)

Details:

Arctica operated at 200 kV with a GatanK3 imaging system collected at 36,000X nominal magnification. The calibrated pixel size of 0.5480 Å was used for processing.
Movies were collected using Leginon (Suloway et al., 2005) at a dose rate of 21.00 e-/Å2/s with a total exposure of 2.40 seconds, for an accumulated dose of 50.40 e-/Å2. Intermediate frames were recorded every 0.05 seconds for a total of 48 frames per micrograph.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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