EMPIAR-11048 - Cryo-EM structure of human NKCC1 K289NA492E bound with bumetanide [4273 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11048

Cryo-EM structure of human NKCC1 K289NA492E bound with bumetanide [4273 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide

Zhao Y, Roy K, Vidossich P, Cancedda L, De Vivo M, Forbush B, Cao E

Nature communications 13 (2022)

PMID: 35585053

DOI: 10.1038/s41467-022-30407-3

Related EMDB entry:

EMD-24812

Deposited:

2022-03-07

Released:

2022-05-31

Last modified:

2023-02-07

Imageset size:

2.28 TB

Imageset DOI:

10.6019/EMPIAR-11048

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM structure of human NKCC1 K289NA492E bound with bumetanide

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4273

Image size:

(11528, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.53, 0.53)

Details:

---

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Citations

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Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

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