EMPIAR-10912 - Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha1, beta3 and gamma subunits, in presence of Ro15-4513 and megabody Mb38 [689 multi-frame micrographs composed of 65 frames each in MRC format]

EMPIAR-10912

Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha1, beta3 and gamma subunits, in presence of Ro15-4513 and megabody Mb38 [689 multi-frame micrographs composed of 65 frames each in MRC format]

Publication:

Differential assembly diversifies GABA_A receptor structures and signalling

Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR

Nature (2022)

PMID: 35355020

DOI: 10.1038/s41586-022-04517-3

Related EMDB entry:

EMD-14076

Deposited:

2021-12-29

Released:

2022-04-04

Last modified:

2022-04-04

Imageset size:

1.50 TB

Imageset DOI:

10.6019/EMPIAR-10912

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of GABA(A)Rs purified from cells expressing human full-length alpha1, beta3 and gamma subunits, in presence of Ro15-4513 and megabody Mb38

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

689

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.06, 1.06)

Details:

Falcon 3 movies with a total dose of 28 e/A2 collected with a Volta Phase Plate.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Cryo-EM Structure Reconstruction by Gaussian Splatting: Pushing the Resolution to Extreme

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2025)

Molecular exaptation by the integrin αI domain.

Hollis JA, Chan MC, Malik HS, Campbell MG. (2025)

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