EMPIAR-10688 - Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing WT PopZ [multiple data sets in MRC and TIFF formats]

EMPIAR-10688

Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing WT PopZ [multiple data sets in MRC and TIFF formats]

Publication:

The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems

Lasker K, Boeynaems S, Lam V, Scholl D, Stainton E, Briner A, Jacquemyn M, Daelemans D, Deniz A, Villa E, Holehouse AS, Gitler AD, Shapiro L

Nature communications 13 (2022)

PMID: 36163138

DOI: 10.1038/s41467-022-33221-z

Related EMDB entry:

EMD-23622

Deposited:

2021-04-07

Released:

2022-11-28

Last modified:

2022-11-28

Imageset size:

7.60 GB

Imageset DOI:

10.6019/EMPIAR-10688

Experimental metadata:

Download xml json

Contains:

tilt series
micrographs - multiframe

Image Sets

1. Unaligned tilt series of FIB-milled Caulobacter crescentus expressing WT PopZ

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.33, 4.33)

Details:

Tilt-series is motion-corrected using Warp.

Files:

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2. Multi-frame micrographs of each tilt for tilt-series of FIB-milled Caulobacter crescentus expressing WT PopZ

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.33, 4.33)

Details:

Dark-subtracted frames from serialEM. mdoc metadata file and counting mode gain-reference is included in the same directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

See all citations

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